ABSTRACT
Objective To investigate the effect of valsartan on the proliferation and migration of rat aortic smooth muscle cells and the expression of p-ERK1/2 ,p-P38 promoted by angiotensinⅡ (AngⅡ ) .Methods VSMCs from the rat thoracic aorta were cultured by attachment-block culture .VSMCs from3-5 passages were used .The proliferation of VSMCs was tested by MTT meth-od .Pipettetip wounding injury was used to access the migration of VSMCs .The expression of ERK1/2 ,p-ERK1/2 ,P38 and p-P38 of VSMCs was detected by Western Blot .Results (1) AngⅡ could significantly promote the proliferation of VSMCs .The prolifer-ation of VSMCs promoted by AngⅡ could be inhibited by Valsartan and PD98059 .Valsartan inhibited the proliferation of VSMCs in a concentration-dependence .SB23015 could significantly promote the proliferation of VSMCs .(2 ) The migration activity of VSMCs in the Ang Ⅱ was increased significantly .Valsartan and PD98059 inhibited Ang Ⅱ-induced migration of rat VSMCs and SB23015 was on the contrary .(3) Valsartan and PD98059 could inhibit AngⅡ-induced VSMCs intracellular p-ERK1/2 protein ex-pression ,and this role was enhanced by SB23015 .Valsartan and SB23015 could inhibit Ang Ⅱ-induced VSMCs intracellular p-P38 protein expression ,and PD98059 had no significant effect .(4)VSMCs had no significant effect on cell proliferation ,migration and p-ERK1/2 ,p-P38 protein expression under valsartan alone .Conclusion (1)Valsartan could inhibit Ang Ⅱ-induced proliferation and migration of VSMCs in a concentration-dependence .(2)The effects that valsartan inhibits the proliferation and migration of Ang Ⅱ-induced VSMCs might be related to its inhibiting Ang Ⅱ-induced p-ERK1/2 protein expression .(3)p-P38 might play a negative reg-ulatory role on the AngⅡ-induced p-ERK1/2 activation .
ABSTRACT
ObjectiveTo analyse the clinical characteristics of elderly patients with permanent cardiac pacemaker implanted. MethodsTo retrospectively analyze 41 elderly patients( ≥80 years) with permanent pacemakers implantation. Observing the clinical symptoms, bradyarrhythmia category, pacing mode, intraoperative pacing parameters,intraoperative and postoperative complications. ResultsAll patients were operated successfully. Pacing modes were,VVI 29 cases,VVIR 2 cases,DDD 9 cases and DDDR 1 case. Ventricular electrode located in right ventricular apical pacing. Atrial electrode located right heart ear. During the operateing, severe complications did not happen such as heart perforation, malignant arrhythmia. One case of postoperative dislocation electrode and one case of pocket hematocele were observed. After treatment, they were cured, no infection and pacemaker syndrome happened. 6 patients died during follow-up, postoperative from two months to 6 years. ConclusionPacing therapy in elderly patients is good. Surgical risk was not increased.